Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using DNA Polymerase beta antibody (A1681) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] DNA Polymerase beta Rabbit pAb
Catalog No:AB-A1681
Manufacturer:ABclonal
Synonyms:POLB
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] DNA Polymerase beta Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human DNA Polymerase beta (NP_002681.1). |
Gene ID: | 5423 |
Swiss prot: | P06746 |
Molecular weight: | 38kDa |
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