Western blot analysis of extracts of various cell lines, using XRCC1 antibody (A0442) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
XRCC1 Rabbit pAb
Catalog No:AB-A0442
Manufacturer:ABclonal
Synonyms:XRCC1;RCC
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | XRCC1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human XRCC1 (NP_006288.2). |
Gene ID: | 7515 |
Swiss prot: | P18887 |
Molecular weight: | 69kDa |
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