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Western blot analysis of extracts of various cell lines, using USP18 antibody (A16739) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
USP18 Rabbit pAb
Catalog No：AB-A16739
Manufacturer：ABclonal
Synonyms：USP18;ISG43;UBP43;PTORCH2
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | USP18 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human USP18 (NP_059110.2). |
| Gene ID: | 11274 |
| Swiss prot: | Q9UMW8 |
| Molecular weight: | 43kDa |
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