Western blot analysis of extracts of various cell lines, using MAPK1/MAPK3 antibody (A16736) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
MAPK1/MAPK3 Rabbit pAb
Catalog No:AB-A16736
Manufacturer:ABclonal
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MAPK1/MAPK3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. |
Immunogen: | Recombinant Protein of human MAPK1/MAPK3 |
Gene ID: | 5594/5595 |
Swiss prot: | P28482/P27361 |
Molecular weight: | 36kDa/38kDa/40kDa/41kDa/43kDa |
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