Western blot analysis of extracts of various cell lines, using TFAP4 antibody (A16727) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Western blot analysis of extracts from normal (control) and TFAP4 knockout (KO) HeLa cells, using TFAP4 antibody (A16727) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60S.
[KO Validated] TFAP4 Rabbit pAb
Catalog No:AB-A16727
Manufacturer:ABclonal
Synonyms:TFAP4;AP-4;bHLHc41
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] TFAP4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009] |
Immunogen: | A synthetic Peptide of human TFAP4 |
Gene ID: | 7023 |
Swiss prot: | Q01664 |
Molecular weight: | 38kDa |
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