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Western blot analysis of extracts of various cell lines, using PDPK1 antibody (A1665) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and PDPK1 knockout (KO) HCT116 cells, using PDPK1 antibody (A1665) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using PDPK1 antibody (A1665) at dilution of 1:200 (40x lens).
[KO Validated] PDPK1 Rabbit pAb
Catalog No：AB-A1665
Manufacturer：ABclonal
Synonyms：PDPK1;PDK1;PDPK2;PDPK2P;PRO0461
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] PDPK1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | PDPK1 (3-phosphoinositide dependent protein kinase 1) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity and insulin receptor binding. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1 (NP_112558.2). |
| Gene ID: | 5170 |
| Swiss prot: | O15530 |
| Molecular weight: | 48kDa/50kDa/58kDa/60kDa/63kDa |
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