Western blot analysis of extracts of various cell lines, using BAF250 antibody (A16648) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
BAF250 Rabbit pAb
Catalog No:AB-A16648
Manufacturer:ABclonal
Synonyms:ARID1A;B120;BAF250;BAF250a;BM029;C1orf4;CSS2;ELD;MRD14;OSA1;P270;SMARCF1;hELD;hOSA1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BAF250 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of mouse BAF250 (NP_001074288.1). |
Gene ID: | 93760 |
Swiss prot: | A2BH40 |
Molecular weight: | 206kDa/218kDa/242kDa |
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