Western blot analysis of extracts of various cell lines, using PD-L1/CD274 antibody (A1645) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts from normal (control) and PD-L1/CD274 knockout (KO) A-549 cells, using PD-L1/CD274 antibody (A1645) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
PD-L1/CD274 Rabbit pAb
Catalog No:AB-A1645
Manufacturer:ABclonal
Synonyms:B7-H;B7H1;PDL1;PD-L1;PDCD1L1;PDCD1LG1;CD274
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PD-L1/CD274 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-238 of human PD-L1/CD274 (NP_054862.1). |
Gene ID: | 29126 |
Swiss prot: | Q9NZQ7 |
Molecular weight: | 20kDa/33kDa |
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