Western blot analysis of extracts of SH-SY5Y cells, using Neurofilament M Rabbit pAb (A16405) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Neurofilament M Rabbit pAb
Catalog No:AB-A16405
Manufacturer:ABclonal
Synonyms:NEF3;NF-M;NFM;NEFM
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Neurofilament M Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Neurofilament M (NP_005373.2). |
Gene ID: | 4741 |
Swiss prot: | P07197 |
Molecular weight: | 59kDa/102kDa |
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