Western blot analysis of extracts of various cell lines, using MTIF2 antibody (A16402) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
MTIF2 Rabbit pAb
Catalog No:AB-A16402
Manufacturer:ABclonal
Synonyms:MTIF2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MTIF2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2). |
Gene ID: | 4528 |
Swiss prot: | P46199 |
Molecular weight: | 81kDa |
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