Western blot analysis of extracts of RAW264.7 cells, using iNOS antibody (A0312) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
iNOS Rabbit pAb
Catalog No:AB-A0312
Manufacturer:ABclonal
Synonyms:NOS;INOS;NOS2A;HEP-NOS;iNOS;NOS2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | iNOS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human iNOS (NP_000616.3). |
Gene ID: | 4843 |
Swiss prot: | P35228 |
Molecular weight: | 126kDa/131kDa |
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