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Western blot analysis of extracts of HeLa cells, using HSP27/HSP27/HSPB1 antibody (A16332) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts from normal (control) and HSP27/HSP27/HSPB1 knockout (KO) HeLa cells, using HSP27/HSP27/HSPB1 antibody (A16332) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human breast cancer using HSP27/HSP27/HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using HSP27/HSP27/HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using HSP27/HSP27/HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using HSP27/HSP27/HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
[KO Validated] HSP27/HSPB1 Rabbit pAb
Catalog No：AB-A16332
Manufacturer：ABclonal
Synonyms：HSPB1;CMT2F;HEL-S-102;HMN2B;HS.76067;HSP27;HSP28;Hsp25;SRP27
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HSP27/HSPB1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSP27/HSP27/HSPB1 (NP_001531.1). |
| Gene ID: | 3315 |
| Swiss prot: | P04792 |
| Molecular weight: | 22kDa |
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