Western blot analysis of extracts of various cell lines, using PPP1R15A antibody (A16260) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PPP1R15A Rabbit pAb
Catalog No:AB-A16260
Manufacturer:ABclonal
Synonyms:PPP1R15A;GADD34
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PPP1R15A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human PPP1R15A (NP_055145.3). |
Gene ID: | 23645 |
Swiss prot: | O75807 |
Molecular weight: | 49kDa/50kDa 56kDa/73kDa |
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