.jpg)
Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 Rabbit pAb (A0290) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 Rabbit pAb (A0290) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded rat brain using CDKN1B/p27KIP1 Antibody (A0290) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts from normal (control) and CDKN1B/p27KIP1 knockout (KO) HeLa cells, using CDKN1B/p27KIP1 antibody (A0290) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat brain using CDKN1B/p27KIP1 Antibody (A0290) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CDKN1B/p27KIP1 Antibody (A0290) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using CDKN1B/p27KIP1 Antibody (A0290) at dilution of 1:100 (40x lens).
[KO Validated] CDKN1B/p27KIP1 Rabbit pAb
Catalog No：AB-A0290
Manufacturer：ABclonal
Synonyms：CDKN1B;CDKN4;KIP1;MEN1B;MEN4;P27KIP1;p27 KIP 1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] CDKN1B/p27KIP1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1). |
| Gene ID: | 1027 |
| Swiss prot: | P46527 |
| Molecular weight: | 22kDa |
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