Western blot analysis of extracts of various cell lines, using D2HGDH antibody (A16213) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
D2HGDH Rabbit pAb
Catalog No:AB-A16213
Manufacturer:ABclonal
Synonyms:D2HGDH;D2HGD
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | D2HGDH Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 322-521 of human D2HGDH (NP_689996.4). |
Gene ID: | 728294 |
Swiss prot: | Q8N465 |
Molecular weight: | 32kDa/34kDa/56kDa |
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