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Western blot analysis of extracts of A-549 cells, using IDO1 antibody (A1614) at 1:500 dilution.A549 cells were treated by hIFN-γ（100ng/mL） at 37℃ for 48 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
IDO1 Rabbit pAb
Catalog No：AB-A1614
Manufacturer：ABclonal
Synonyms：IDO1;IDO;IDO-1;INDO;indoleamine 2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | IDO1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes indoleamine 2,3-dioxygenase (IDO) – a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-403 of human IDO1 (NP_002155.1). |
| Gene ID: | 3620 |
| Swiss prot: | P14902 |
| Molecular weight: | 45kDa |
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