Western blot analysis of extracts of various cell lines, using TNF-α antibody (A0277) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
TNF-α Rabbit pAb
Catalog No:AB-A0277
Manufacturer:ABclonal
Synonyms:TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €1.760,00
Product Name: | TNF-α Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF (NP_000585.2). |
Gene ID: | 7124 |
Swiss prot: | P01375 |
Molecular weight: | 25kDa |
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