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Western blot analysis of extracts of various cell lines, using APEX2 antibody (A16116) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
APEX2 Rabbit pAb
Catalog No：AB-A16116
Manufacturer：ABclonal
Synonyms：APEX2;APE2;APEXL2;XTH2;ZGRF2
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | APEX2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APEX2 (NP_055296.2). |
| Gene ID: | 27301 |
| Swiss prot: | Q9UBZ4 |
| Molecular weight: | 57kDa |
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