Western blot analysis of extracts of various cell lines, using MED7 antibody (A16088) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
MED7 Rabbit pAb
Catalog No:AB-A16088
Manufacturer:ABclonal
Synonyms:MED7;ARC34;CRSP33;CRSP9
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MED7 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human MED7 (NP_004261.1). |
Gene ID: | 9443 |
Swiss prot: | O43513 |
Molecular weight: | 27kDa |
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