Western blot analysis of extracts of various cell lines, using MTA1 antibody (A16085) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
MTA1 Rabbit pAb
Catalog No:AB-A16085
Manufacturer:ABclonal
Synonyms:MTA1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MTA1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human MTA1 (NP_004680.2). |
Gene ID: | 9112 |
Swiss prot: | Q13330 |
Molecular weight: | 49kDa/79kDa/80kDa |
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