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Western blot analysis of extracts of various cell lines, using Neurofilament L antibody (A0257) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
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Immunohistochemistry of paraffin-embedded human breast cancer using Neurofilament L antibody (A0257) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using Neurofilament L antibody (A0257) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using Neurofilament L antibody (A0257) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using Neurofilament L antibody (A0257) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using Neurofilament L antibody (A0257) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Neurofilament L antibody (A0257) at dilution of 1:200 (40x lens).
Neurofilament L Rabbit pAb
Catalog No：AB-A0257
Manufacturer：ABclonal
Synonyms：NEFL;CMT1F;CMT2E;NF-L;NF68;NFL;PPP1R110
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Neurofilament L Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-543 of human Neurofilament L (NP_006149.2). |
| Gene ID: | 4747 |
| Swiss prot: | P07196 |
| Molecular weight: | 61kDa |
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