Western blot analysis of extracts of various cell lines, using UBE2J1 antibody (A15841) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
UBE2J1 Rabbit pAb
Catalog No:AB-A15841
Manufacturer:ABclonal
Synonyms:UBE2J1;CGI-76;HSPC153;HSPC205;HSU93243;NCUBE-1;NCUBE1;UBC6;UBC6E;Ubc6p
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UBE2J1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2). |
Gene ID: | 51465 |
Swiss prot: | Q9Y385 |
Molecular weight: | 35kDa |
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