Western blot analysis of extracts of various cell lines, using NDUFAF1 antibody (A15835) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 35s.
NDUFAF1 Rabbit pAb
Catalog No:AB-A15835
Manufacturer:ABclonal
Synonyms:NDUFAF1;CGI-65;CGI65;CIA30
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NDUFAF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2). |
Gene ID: | 51103 |
Swiss prot: | Q9Y375 |
Molecular weight: | 37kDa |
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