Western blot analysis of extracts of various cell lines, using UNC13B antibody (A15780) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
UNC13B Rabbit pAb
Catalog No:AB-A15780
Manufacturer:ABclonal
Synonyms:UNC13B;MUNC13;UNC13;Unc13h2
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UNC13B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3). |
Gene ID: | 10497 |
Swiss prot: | O14795 |
Molecular weight: | 180kDa/182kDa |
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