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Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (A15641) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] RhoA Rabbit pAb (A15641) at dilution of 1:350 (40x lens).
[KO Validated] RhoA Rabbit pAb
Catalog No：AB-A15641
Manufacturer：ABclonal
Synonyms：ARH12;ARHA;RHO12;RHOH12;Rho;RHOA;RhoA
Applications：WB,IHC,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] RhoA Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoA (NP_001655.1). |
| Gene ID: | 387 |
| Swiss prot: | P61586 |
| Molecular weight: | 21kDa |
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