Western blot analysis of extracts of various cell lines, using Angiotensinogen antibody (A15637) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Angiotensinogen Rabbit pAb
Catalog No:AB-A15637
Manufacturer:ABclonal
Synonyms:AGT;ANHU;hFLT1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Angiotensinogen Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human Angiotensinogen (NP_000020.1). |
Gene ID: | 183 |
Swiss prot: | P01019 |
Molecular weight: | 53kDa |
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