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Western blot analysis of extracts of various cell lines, using ITCH antibody (A15612) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts from normal (control) and ITCH knockout (KO) 293T cells, using ITCH antibody (A15612) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded human lung cancer using ITCH antibody (A15612) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using ITCH antibody (A15612) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ITCH antibody (A15612) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ITCH antibody (A15612) at dilution of 1:100 (40x lens).
[KO Validated] ITCH Rabbit pAb
Catalog No：AB-A15612
Manufacturer：ABclonal
Synonyms：ITCH;ADMFD;AIF4;AIP4;NAPP1
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] ITCH Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ITCH (NP_001244066.1). |
| Gene ID: | 83737 |
| Swiss prot: | Q96J02 |
| Molecular weight: | 103kDa |
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Catalog No：AB-A0951
Manufacturer：ABclonal
Synonyms：FOX-3;FOX3;HRNBP3;NEUN;NeuN;RBFOX3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €1.170,00
Catalog No：AB-A0887
Manufacturer：ABclonal
Synonyms：BAF190;BAF190A;BRG1;CSS4;MRD16;RTPS2;SNF2;SNF2L4;SNF2LB;SWI2;hSNF2b;SMARCA4
Applications：WB,IHC,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
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Manufacturer：ABclonal
Synonyms：MECP2;AUTSX3;MRX16;MRX79;MRXS13;MRXSL;PPMX;RS;RTS;RTT
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
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Catalog No：AB-A0290
Manufacturer：ABclonal
Synonyms：CDKN1B;CDKN4;KIP1;MEN1B;MEN4;P27KIP1;p27 KIP 1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
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Manufacturer：ABclonal
Synonyms：ERK;ERK-2;ERK2;ERT1;MAPK2;P42MAPK;PRKM1;PRKM2;p38;p40;p41;p41mapk;p42-MAPK;MAPK1
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
Catalog No：AB-A0207
Manufacturer：ABclonal
Synonyms：BCL2L4;BAX
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00