Western blot analysis of extracts of various cell lines, using acetyl-ACC1 antibody (A15606) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
acetyl-ACC1 Rabbit pAb
Catalog No:AB-A15606
Manufacturer:ABclonal
Synonyms:ACACA;ACAC;ACACAD;ACC;ACC1;ACCA
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | acetyl-ACC1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5′ sequence and encoding distinct isoforms have been found for this gene. |
Immunogen: | A synthetic peptide of human acetyl-CoA carboxylase alpha. |
Gene ID: | 31 |
Swiss prot: | Q13085 |
Molecular weight: | 257kDa/259kDa/265kDa/269kDa |
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