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Western blot analysis of extracts of various cell lines, using Twist antibody (A15596) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and Twist knockout (KO) 293T cells, using Twist antibody (A15596) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat liver using Twist Rabbit pAb (A15596) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using Twist Rabbit pAb (A15596) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using Twist Rabbit pAb (A15596) at dilution of 1:200 (40x lens).
[KO Validated] Twist Rabbit pAb
Catalog No：AB-A15596
Manufacturer：ABclonal
Synonyms：TWIST1;ACS3;BPES2;BPES3;CRS;CRS1;CSO;SCS;TWIST;bHLHa38
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] Twist Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Twist (NP_000465.1). |
| Gene ID: | 7291 |
| Swiss prot: | Q15672 |
| Molecular weight: | 20kDa |
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