Western blot analysis of extracts of various cell lines, using Bmi1 Rabbit pAb (A0211) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using Bmi1 Rabbit pAb (A0211) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Bmi1 Rabbit pAb
Catalog No:AB-A0211
Manufacturer:ABclonal
Synonyms:BMI1;FLVI2/BMI1;PCGF4;RNF51;flvi-2/bmi-1;Bmi1
Applications:WB,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Bmi1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human Bmi1 (NP_005171.4). |
Gene ID: | 648 |
Swiss prot: | P35226 |
Molecular weight: | 36kDa |
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