Western blot analysis of extracts of various cell lines, using DNAJC24 antibody (A15560) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
DNAJC24 Rabbit pAb
Catalog No:AB-A15560
Manufacturer:ABclonal
Synonyms:DNAJC24;DPH4;JJJ3;ZCSL3
Applications:WB
Reactivity:Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DNAJC24 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2-149 of human DNAJC24 (NP_859057.4). |
Gene ID: | 120526 |
Swiss prot: | Q6P3W2 |
Molecular weight: | 9kDa/17kDa |
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