Western blot analysis of extracts of various cell lines, using MAF1 antibody (A15531) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
MAF1 Rabbit pAb
Catalog No:AB-A15531
Manufacturer:ABclonal
Synonyms:MAF1;MAF1 homolog
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MAF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MAF1 (NP_115648.2). |
Gene ID: | 84232 |
Swiss prot: | Q9H063 |
Molecular weight: | 28kDa |
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