Western blot analysis of extracts of THP-1 cells, using Bak antibody (A0204) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Bak Rabbit pAb
Catalog No:AB-A0204
Manufacturer:ABclonal
Synonyms:BAK1;BAK;BAK-LIKE;BCL2L7
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Bak Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 62-112 of human Bak (NP_001179.1). |
Gene ID: | 578 |
Swiss prot: | Q16611 |
Molecular weight: | 16kDa/23kDa |
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