Western blot analysis of extracts of various cell lines, using Rad50 antibody (A0182) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 10s.
Rad50 Rabbit pAb
Catalog No:AB-A0182
Manufacturer:ABclonal
Synonyms:RAD50;NBSLD;RAD502;hRad50
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Rad50 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder. |
Immunogen: | A synthetic peptide of human Rad50 |
Gene ID: | 10111 |
Swiss prot: | Q92878 |
Molecular weight: | 138kDa/153kDa/154kDa |
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