Western blot analysis of extracts of various cell lines, using TCIRG1 antibody (A15382) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
TCIRG1 Rabbit pAb
Catalog No:AB-A15382
Manufacturer:ABclonal
Synonyms:TCIRG1;ATP6N1C;ATP6V0A3;Atp6i;OC-116kDa;OC116;OPTB1;Stv1;TIRC7;Vph1;a3
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TCIRG1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TCIRG1 (NP_006010.2). |
Gene ID: | 10312 |
Swiss prot: | Q13488 |
Molecular weight: | 68kDa/92kDa |
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