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Western blot analysis of extracts of various cell lines, using PPM1F Rabbit pAb (A15363) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
PPM1F Rabbit pAb
Catalog No：AB-A15363
Manufacturer：ABclonal
Synonyms：PPM1F;CAMKP;CaMKPase;FEM-2;POPX2;hFEM-2
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PPM1F Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_055449.1). |
| Gene ID: | 9647 |
| Swiss prot: | P49593 |
| Molecular weight: | 38kDa/49kDa |
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