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Western blot analysis of extracts of various cell lines, using RACK1 antibody (A0151) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human oophoroma using RACK1 antibody (A0151) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using RACK1 antibody (A0151). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human oophoroma using RACK1 antibody (A0151) at dilution of 1:100 (40x lens).
RACK1 Rabbit pAb
Catalog No：AB-A0151
Manufacturer：ABclonal
Synonyms：RACK1;GNB2L1;Gnb2-rs1;H12.3;HLC-7;PIG21
Applications：WB,IHC,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | RACK1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules.Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-290 of human RACK1 (NP_006089.1). |
| Gene ID: | 10399 |
| Swiss prot: | P63244 |
| Molecular weight: | 35kDa |
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