Western blot analysis of extracts of various cell lines, using HLA-DOA antibody (A15277) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90S.
HLA-DOA Rabbit pAb
Catalog No:AB-A15277
Manufacturer:ABclonal
Synonyms:HLA-DOA;HLA-DNA;HLA-DZA;HLADZ;major histocompatibility complex;class II;DO alpha
Applications:WB
Reactivity:Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-DOA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human HLA-DOA (NP_002110.1). |
Gene ID: | 3111 |
Swiss prot: | P06340 |
Molecular weight: | 27kDa |
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