Western blot analysis of extracts of various cell lines, using CENPE Rabbit pAb (A15263) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CENPE Rabbit pAb
Catalog No:AB-A15263
Manufacturer:ABclonal
Synonyms:CENPE;CENP-E;KIF10;MCPH13;PPP1R61
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CENPE Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2542-2701 of human CENPE (NP_001804.2). |
Gene ID: | 1062 |
Swiss prot: | Q02224 |
Molecular weight: | 301kDa/312kDa/316kDa |
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