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Western blot analysis of extracts of Mouse brain, using FGD5 antibody (A15212) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
FGD5 Rabbit pAb
Catalog No：AB-A15212
Manufacturer：ABclonal
Synonyms：FGD5;ZFYVE23;FYVE
Applications：WB
Reactivity：Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | FGD5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | FGD5 (or ZFYVE23) is an endothelial specific gene, plays a crucial role in vascular pruning. Deficient expression of FGD5 markedly inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model. FGD5-deficient endothelial cells fail to migrate. Moreover, the cells are susceptible to proapoptotic stimuli associated with VEGF-stimulated activation of Akt. FGD5 promotes apoptosis-induced vaso-obliteration via induction of the hey1-p53 pathway by direct binding and activation of CDC42. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human FGD5 (NP_689749.3). |
| Gene ID: | 152273 |
| Swiss prot: | Q6ZNL6 |
| Molecular weight: | 61kDa/159kDa |
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