Western blot analysis of extracts of various cell lines, using PRR5 antibody (A15170) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using PRR5 antibody (A15170) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PRR5 Rabbit pAb
Catalog No:AB-A15170
Manufacturer:ABclonal
Synonyms:PRR5;FLJ20185k;PP610;PROTOR-1;PROTOR1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PRR5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 109-388 of human PRR5 (NP_851850.1). |
Gene ID: | 55615 |
Swiss prot: | P85299 |
Molecular weight: | 31kDa/32kDa/41kDa/42kDa/45kDa |
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