Western blot analysis of extracts of various cell lines, using RNASEH2A antibody (A15132) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
RNASEH2A Rabbit pAb
Catalog No:AB-A15132
Manufacturer:ABclonal
Synonyms:RNASEH2A;AGS4;JUNB;RNASEHI;RNHIA;RNHL
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RNASEH2A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human RNASEH2A (NP_006388.2). |
Gene ID: | 10535 |
Swiss prot: | O75792 |
Molecular weight: | 33kDa |
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