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Western blot analysis of extracts of various cell lines, using EIF5B antibody (A15123).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
EIF5B Rabbit pAb
Catalog No：AB-A15123
Manufacturer：ABclonal
Synonyms：EIF5B;IF2
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | EIF5B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3). |
| Gene ID: | 9669 |
| Swiss prot: | O60841 |
| Molecular weight: | 138kDa |
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