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Western blot analysis of extracts of various cell lines, using B-Raf antibody (A15033) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat heart using B-Raf Rabbit pAb (A15033) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver using B-Raf Rabbit pAb (A15033) at dilution of 1:100 (40x lens).
B-Raf Rabbit pAb
Catalog No：AB-A15033
Manufacturer：ABclonal
Synonyms：BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | B-Raf Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-449 of human B-Raf (NP_004324.2). |
| Gene ID: | 673 |
| Swiss prot: | P15056 |
| Molecular weight: | 84kDa |
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