Western blot analysis of extracts of various cell lines, using AARS Rabbit pAb (A15017) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
AARS Rabbit pAb
Catalog No:AB-A15017
Manufacturer:ABclonal
Synonyms:AARS;CMT2N;EIEE29
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AARS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AARS (NP_001596.2). |
Gene ID: | 16 |
Swiss prot: | P49588 |
Molecular weight: | 106kDa/109kDa |
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