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Western blot analysis of extracts of various cell lines, using JAM3 antibody (A14942) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
JAM3 Rabbit pAb
Catalog No：AB-A14942
Manufacturer：ABclonal
Synonyms：JAM3;JAM-2;JAM-3;JAM-C;JAMC
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | JAM3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JAM3 (NP_116190.3). |
| Gene ID: | 83700 |
| Swiss prot: | Q9BX67 |
| Molecular weight: | 29kDa/35kDa |
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