Western blot analysis of extracts of various cell lines, using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN)) antibody (A0053) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using Thioredoxin 1 (Trx1/TXN) antibody (A0053) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of Rat liver, using Thioredoxin 1 (Trx1/TXN) antibody (A0053) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Thioredoxin 1 (Trx1/TXN) Rabbit pAb
Catalog No:AB-A0053
Manufacturer:ABclonal
Synonyms:TXN;TRDX;TRX;TRX1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Thioredoxin 1 (Trx1/TXN) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN)) (NP_003320.2). |
Gene ID: | 7295 |
Swiss prot: | P10599 |
Molecular weight: | 9kDa/11kDa |
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