Western blot analysis of extracts of various cell lines, using PABPC1 antibody (A14872) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
pAbPC1 Rabbit pAb
Catalog No:AB-A14872
Manufacturer:ABclonal
Synonyms:PABPC1;PAB1;PABP;PABP1;PABPC2;PABPL1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | pAbPC1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3′ poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2). |
Gene ID: | 26986 |
Swiss prot: | P11940 |
Molecular weight: | 61kDa/70kDa |
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