Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0010) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0010) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
PARP1 Rabbit pAb
Catalog No:AB-A0010
Manufacturer:ABclonal
Synonyms:ADPRT;ADPRT 1;ADPRT1;ARTD1;PARP;PARP-1;PPOL;pADPRT-1;PARP1;Cleaved PARP;PARP1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €605,00
Product Name: | PARP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2). |
Gene ID: | 142 |
Swiss prot: | P09874 |
Molecular weight: | 113kDa |
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