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Western blot analysis of extracts of various cell lines, using PHD2 antibody (A14557) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using PHD2 antibody (A14557) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
.jpg)
Western blot analysis of extracts from normal (control) and PHD2 knockout (KO) 293T cells, using PHD2 antibody (A14557) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded Rat kidney using PHD2 antibody (A14557) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human colon using PHD2 antibody (A14557) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse brain using PHD2 antibody (A14557) at dilution of 1:100 (40x lens).
[KO Validated] PHD2 Rabbit pAb
Catalog No：AB-A14557
Manufacturer：ABclonal
Synonyms：EGLN1;C1orf12;ECYT3;HALAH;HIF-PH2;HIFPH2;HPH-2;HPH2;PHD2;SM20;ZMYND6
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] PHD2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2 (NP_071334.1). |
| Gene ID: | 54583 |
| Swiss prot: | Q9GZT9 |
| Molecular weight: | 36kDa/43kDa/46kDa |
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